Contact Information

Email Steven D Goodman, PhD

      700 Children's DriveColumbus, OH 43205 (map)

Learn more about Steven D Goodman

Research

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Biography

        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Professional Experience

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

Contact Information

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Email Steven D Goodman, PhD

      700 Children's DriveColumbus, OH 43205 (map)

Learn more about Steven D Goodman

Research

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Biography

        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Professional Experience

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

Contact Information

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Email Steven D Goodman, PhD

      700 Children's DriveColumbus, OH 43205 (map)

Learn more about Steven D Goodman

Contact Information

  • Email Steven D Goodman, PhD
  • 700 Children’s DriveColumbus, OH 43205 (map)

Learn more about Steven D Goodman

Research

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Research

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Research

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Lab(s)

Center for Microbial Pathogenesis

View My Publications Publications

                  Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.

                


                  Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  

                


                  Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 

                


                  Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.

                


                  Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.

                


                  Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.

                


                  Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.

                


                  Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  

                


                  Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 

                


                  Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 

                


                  Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.

                


                  Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 

                


                  Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.

                


                  Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.

                


                  Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 

                


                  Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.

                


                  Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.

                


                  Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.

                


                  Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.

                


                  Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.

                


                  Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.

                


                  Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  

                


                  Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 

                


                  Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

Lab(s)

Center for Microbial Pathogenesis

View My Publications

Lab(s)

Center for Microbial Pathogenesis

  • Center for Microbial Pathogenesis

                    Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.



                Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;  



                Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10: 



                Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10;  AAC0187721.



                Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.



                Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.



                Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.



                Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;  



                Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131: 



                Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14: 



                Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.



                Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13: 



                Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.



                Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.



                Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.



                Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5: 



                Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.



                Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.



                Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.



                Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20;  1-9.



                Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.



                Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.



                Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;  



                Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200: 



                Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

View More Publications

  • Jurcisek JA, Hofer LK, Goodman SD, Bakaletz LO. Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms. Biofilm. 2022 Dec; 4: 100096.
  • Chang HL, Su KY, Goodman SD, Cheng WC, Lin LI, Yang YC, Chang SY, Fang WH. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J Vis Exp. 2022 Apr 22;
  • Goodman SD, Bakaletz LO. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be Targeted for Biofilm Resolution. Microorganisms. 2022 Feb 18; 10:
  • Kurbatfinski N, Goodman SD, Bakaletz LO. A Humanized Monoclonal Antibody Potentiates Killing by Antibiotics of Diverse Biofilm-Forming Respiratory Tract Pathogens. Antimicrob Agents Chemother. 2022 Jan 10; AAC0187721.
  • Ragan MV, Wala SJ, Goodman SD, Bailey MT, Besner GE. Next-Generation Probiotic Therapy to Protect the Intestines From Injury. Front Cell Infect Microbiol. 2022; 12: 863949.
  • Buzzo JR, Devaraj A, Gloag ES, Jurcisek JA, Robledo-Avila F, Kesler T, Wilbanks K, Mashburn-Warren L, Balu S, Wickham J, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell. 2021 Nov 11; 184: 5740-5758.e17.
  • Novotny LA, Chiang T, Goodman SD, Elmaraghy CA, Bakaletz LO. Humanized Anti-DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media. Laryngoscope. 2021 Oct; 131: E2698-E2704.
  • Shelby RD, Mar P, Janzow GE, Mashburn-Warren L, Tengberg N, Navarro JB, Allen JM, Wickham J, Wang Y, Bailey MT, Goodman SD, Besner GE. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis. J Pediatr Surg. 2021 Sep 20;
  • Devaraj A, Novotny LA, Robledo-Avila FH, Buzzo JR, Mashburn-Warren L, Jurcisek JA, Tjokro NO, Partida-Sanchez S, Bakaletz LO, Goodman SD. The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation. J Clin Invest. 2021 Aug 16; 131:
  • Wang Y, Jaggers RM, Mar P, Galley JD, Shaffer T, Rajab A, Deshpande S, Mashburn-Warren L, Buzzo JR, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats. Brain Behav Immun Health. 2021 Jul; 14:
  • Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental plaque biofilm matrix. Periodontol 2000. 2021 Jun; 86: 32-56.
  • Al-Hadidi A, Navarro J, Goodman SD, Bailey MT, Besner GE. Lactobacillus reuteri in Its Biofilm State Improves Protection from Experimental Necrotizing Enterocolitis. Nutrients. 2021 Mar 12; 13:
  • Shelby RD, Janzow GE, Mashburn-Warren L, Galley J, Tengberg N, Navarro J, Conces M, Bailey MT, Goodman SD, Besner GE. A novel probiotic therapeutic in a murine model of Clostridioides difficile colitis. Gut Microbes. 2020 Nov 9; 12: 1814119.
  • Novotny LA, Goodman SD, Bakaletz LO. Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections. EBioMedicine. 2020 Sep; 59: 102867.
  • Martyn L, Sethia R, Chon R, Novotny L, Goodman SD, Elmaraghy C, Bakaletz LO. Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms. Laryngoscope. 2020 Jun; 130: 1364-1371.
  • Bailey MT, Lauber CL, Novotny LA, Goodman SD, Bakaletz LO. Immunization with a Biofilm-Disrupting Nontypeable Haemophilus influenzae Vaccine Antigen Did Not Alter the Gut Microbiome in Chinchillas, Unlike Oral Delivery of a Broad-Spectrum Antibiotic Commonly Used for Otitis Media. mSphere. 2020 Apr 15; 5:
  • Chang HL, Su KY, Goodman SD, Chou NA, Lin KC, Cheng WC, Lin LI, Chang SY, Fang WH. Proofreading of single nucleotide insertion/deletion replication errors analyzed by MALDI-TOF mass spectrometry assay. DNA Repair (Amst). 2020 Apr; 88: 102810.
  • Barron CL, Kamel-Abusalha LB, Sethia R, Goodman SD, Elmaraghy CA, Bakaletz LO. Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients. Laryngoscope. 2020 Mar; 130: 806-811.
  • Devaraj A, Buzzo JR, Mashburn-Warren L, Gloag ES, Novotny LA, Stoodley P, Bakaletz LO, Goodman SD. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Proc Natl Acad Sci U S A. 2019 Dec 10; 116: 25068-25077.
  • Shelby RD, Tengberg N, Conces M, Olson JK, Navarro JB, Bailey MT, Goodman SD, Besner GE. Development of a Standardized Scoring System to Assess a Murine Model of Clostridium difficile Colitis. J Invest Surg. 2019 Mar 20; 1-9.
  • Novotny LA, Goodman SD, Bakaletz LO. Redirecting the immune response towards immunoprotective domains of a DNABII protein resolves experimental otitis media. NPJ Vaccines. 2019; 4: 43.
  • Mashburn-Warren L, Goodman SD, Federle MJ, Prehna G. The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Sci Rep. 2018 Nov 8; 8: 16535.
  • Su KY, Goodman SD, Lai HM, Yen RS, Hu WY, Cheng WC, Lin LI, Yang YC, Fang WH. Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis. J Vis Exp. 2018 Jun 19;
  • Rocco CJ, Bakaletz LO, Goodman SD. Targeting the HUß Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms. J Bacteriol. 2018 Jun 1; 200:
  • Su KY, Lin LI, Goodman SD, Yen RS, Wu CY, Chang WC, Yang YC, Cheng WC, Fang WH. DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo. DNA Repair (Amst). 2018 Apr; 64: 59-67.

Biography

        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system

Biography

        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system

Biography

        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system



      






        Dr. Goodman is a principal investigator in the Center for Microbial Pathogenesis. His research focuses on gene regulation in biofilms, bacterial surface detection and attachment, horizontal gene transfer (HGT) and metagenomics, and bacterial histones. 1. Gene Regulation in Biofilms: In nature, bacteria are often found in complex microbial ecosystems. In some cases these bacteria associate to form interactive systems commonly referred to as biofilms. The oral cavity is home to hundreds of bacteria. One of these bacteria, Streptococcus mutans, causes dental caries (cavities) and is known to form biofilms (plaque) on the surface of teeth. Critical to the formation of these biofilms are the genes required to adhere to teeth. We have found that the expression of these genes is strongly regulated and coupled to the ability of the bacteria to sense their environment (e.g. when they encounter the surface of a tooth.) One of the ways that bacteria can do this is through Two Component Signal Transduction Systems (TCSTS). These systems are composed of a membrane bound protein that upon sensing a specific environmental signal alters the function of a specific transcription factor. Each TCSTS has a repertoire of genes whose expression is specifically affected upon signal detection. S. mutans has 13 TCSTS and it is our long term goal to clone, express and determine the biological function(s) of each one. In this way, we will be able to determine what S. mutans senses and how it responds. 2. Bacterial Surface Detection and Attachment: In an extension of our work above and in collaboration with Ken Nealson (Earth Sciences, LAS, USC) and Firdaus Udwadia (Aeropsace and Mechanical Engineering, ENG. USC), it has become very clear that bacteria are exquisite sensors of surfaces as they do not attach randomly to a given surface. We are identifying the surface/material properties that the bacteria need to choose where to attach and finding out what genes are involved in the process. 3. Horizontal Gene Transfer (HGT) and Metagenomics: Bacteria can spread and exchange their genetic information in three ways, namely conjugation (bacterial sex), transduction (viral mediated) and transformation (uptake of extracellular DNA). Recently it has become clear that in a given ecological niche HGT occurs so rampantly that bacteria are believed to have access to the largess of genetic information for all of the constituent bacteria (aka the metagenome). We have discovered a bacteriophage that propagates in the oral cavity by both infection with its native host, a periodontal pathogen Aggregatibacter actinomycetemcomintans, and by transformation. We are using this bacteriophage as a model system to determine which routes of HGT are most likely to explain how genes (e.g. those that encode antibiotic resistance) are spread from one bacterium to another. 4. Bacterial Histones: IHF is a small abundant bacterial protein that binds to and bends a specific DNA sequence. IHF always seems to act in an accessory capacity and is known to function in virtually all types of nucleoprotein systems (e.g. replication, gene expression, and recombination). Thus it has been proposed that IHF modulates (activates or represses) the systems where it acts through DNA bending. In this way, it functionally resembles eukaryotic histones. Recently, we have discovered that IHF and a related protein HU play a critical role in the extracellular matrix of pathogenic biofilms.  This matrix protects bacteria from the immune system and antimicrobial therapies and causes chronicity of bacterial infections.  In collaboration with Lauren Bakaletz (Center for Microbial Pathogenesis) we have developed an immunotherapeutic approach to remove these proteins from the biofilm causing the subsequent release of bacteria from their protective shield and making them vulnerable to clearance by both antibiotics and the immune system

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Center for Microbial Pathogenesis

Principal Investigator

  • Center for Microbial Pathogenesis
  • Principal Investigator

Professional Experience

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

Professional Experience

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

Professional Experience

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator2009 - 2010 University of Southern California, Chair, Biomedical Sciences2009 - 2010 University of Southern California, Associate Professor of Biomedical Sciences

2012 - Present Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Principal Investigator

Contact Information

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)

Email Steven D Goodman

                    700 Children's DriveColumbus, OH 43205 (map)
  • Email Steven D Goodman
  • 700 Children’s DriveColumbus, OH 43205 (map)