Contact Information

Call us at: (614) 355-3403

Email John Gunn, PhD

Center for Microbial Pathogenesis700 Children’s DriveColumbus, OH 43205 (map)

Learn more about John Gunn

Research

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Biography

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

Patient Care

Locations

Main Campus of Nationwide Children’s Hospital

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

Awards, Honors & Organizations

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

Education

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Professional Experience

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

Research Funding

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Contact Information

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Connect with John Gunn

Connect on Twitter

Contact Information

Call us at: (614) 355-3403

Email John Gunn, PhD

Center for Microbial Pathogenesis700 Children’s DriveColumbus, OH 43205 (map)

Learn more about John Gunn

Research

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Biography

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

Patient Care

Locations

Main Campus of Nationwide Children’s Hospital

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

Awards, Honors & Organizations

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

Education

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Professional Experience

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

Research Funding

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Contact Information

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Connect with John Gunn

Connect on Twitter

Contact Information

Call us at: (614) 355-3403

Email John Gunn, PhD

Center for Microbial Pathogenesis700 Children’s DriveColumbus, OH 43205 (map)

Learn more about John Gunn

Contact Information

  • Call us at:
  • (614) 355-3403
  • Email John Gunn, PhD
  • Center for Microbial Pathogenesis700 Children’s DriveColumbus, OH 43205 (map)

Learn more about John Gunn

Research

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Research

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Research

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

View My Publications

Publications

                  Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  

                


                  González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 

                


                  Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.

                


                  Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.

                


                  Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.

                


                  Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.

                


                  Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.

                


                  Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.

                


                  Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.

                


                  Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 

                


                  Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.

                


                  González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  

                


                  van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.

                


                  Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.

                


                  Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.

                


                  Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.

                


                  González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 

                


                  Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.

                


                  Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.

                


                  González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

Lab(s)

Center for Microbial Pathogenesis

Dr. Gunn’s laboratory studies Salmonella and Francisella pathogenesis. The research is particularly focused on bacterial biofilms and regulatory systems that control virulence and modifications of the bacterial cell surface. These attributes are examined in the context of acute and chronic infections where they investigate the contribution of gene regulation and biofilm formation on pathogenesis, immune system recognition, and survival within host phagocytic cells. Additionally, the laboratory is developing new vaccine and therapeutic approaches to counteract both Salmonella and Francisella infections. The Gunn lab developed a mouse model for chronic Salmonella infection of the gallbladder and uses this model to study both host and microbial factors that are responsible for establishing and maintaining asymptomatic, persistent infection of this organ.

Lab(s)

Center for Microbial Pathogenesis

  • Center for Microbial Pathogenesis

  • View My Publications

                    Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;  



                González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10: 



                Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17;  e0061922.



                Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.



                Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.



                Vasicek EM, O'Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.



                Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.



                Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.



                Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.



                Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201: 



                Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.



                González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;  



                van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.



                Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.



                Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.



                Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.



                González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76: 



                Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.



                Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.



                González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

View More Publications

  • Brink KR, Hunt MG, Mu AM, Groszman K, Hoang KV, Lorch KP, Pogostin BH, Gunn JS, Tabor JJ. An E. coli display method for characterization of peptide-sensor kinase interactions. Nat Chem Biol. 2022 Dec 8;
  • González JF, Hitt R, Laipply B, Gunn JS. The Effect of the Gallbladder Environment during Chronic Infection on Salmonella Persister Cell Formation. Microorganisms. 2022 Nov 16; 10:
  • Hoang KV, Woolard K, Yang C, Melander C, Gunn JS. Identification of a Host-Targeted Compound to Control Typhoid Fever. Microbiol Spectr. 2022 May 17; e0061922.
  • Woolard KJ, Sandala JL, Melander RJ, Gunn JS, Melander C. Development of small molecules that work cooperatively with ciprofloxacin to clear salmonella biofilms in a chronic gallbladder carriage model. Eur J Med Chem. 2022 Feb 19; 232: 114203.
  • Hahn MM, González JF, Hitt R, Tucker L, Gunn JS. The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo. Infect Immun. 2021 Oct 15; 89: e0031021.
  • Vasicek EM, O’Neal L, Parsek MR, Fitch J, White P, Gunn JS. L-Arabinose Transport and Metabolism in Salmonella Influences Biofilm Formation. Front Cell Infect Microbiol. 2021; 11: 698146.
  • Hahn MM, González JF, Gunn JS. Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes. Front Cell Infect Microbiol. 2021; 11: 683081.
  • Devaraj A, González JF, Eichar B, Thilliez G, Kingsley RA, Baker S, Allard MW, Bakaletz LO, Gunn JS, Goodman SD. Enhanced biofilm and extracellular matrix production by chronic carriage versus acute isolates of Salmonella Typhi. PLoS Pathog. 2021 Jan; 17: e1009209.
  • Harrell JE, Hahn MM, D’Souza SJ, Vasicek EM, Sandala JL, Gunn JS, McLachlan JB. Salmonella Biofilm Formation, Chronic Infection, and Immunity Within the Intestine and Hepatobiliary Tract. Front Cell Infect Microbiol. 2020; 10: 624622.
  • Neiger MR, González JF, Gonzalez-Escobedo G, Kuck H, White P, Gunn JS. Pathoadaptive Alteration of Salmonella Biofilm Formation in Response to the Gallbladder Environment. J Bacteriol. 2019 Jul 15; 201:
  • Aldapa-Vega G, Moreno-Eutimio MA, Berlanga-Taylor AJ, Jiménez-Uribe AP, Nieto-Velazquez G, López-Ortega O, Mancilla-Herrera I, Cortés-Malagón EM, Gunn JS, Isibasi A, Wong-Baeza I, López-Macías C, Pastelin-Palacios R. Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes. Mol Immunol. 2019 Jul; 111: 43-52.
  • González JF, Tucker L, Fitch J, Wetzel A, White P, Gunn JS. Human bile-mediated regulation of Salmonella curli fimbriae. J Bacteriol. 2019 Apr 1;
  • van Hoek ML, Hoang KV, Gunn JS. Two-Component Systems in Francisella Species. Front Cell Infect Microbiol. 2019; 9: 198.
  • Johnson MM, Collier MA, Hoang KV, Pino EN, Graham-Gurysh EG, Gallovic MD, Zahid MSH, Chen N, Schlesinger L, Gunn JS, Bachelder EM, Ainslie KM. In Vivo and Cellular Trafficking of Acetalated Dextran Microparticles for Delivery of a Host-Directed Therapy for Salmonella enterica Serovar Typhi Infection. Mol Pharm. 2018 Nov 5; 15: 5336-5348.
  • Eguale T, Asrat D, Alemayehu H, Nana I, Gebreyes WA, Gunn JS, Engidawork E. Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia. Zoonoses Public Health. 2018 Nov; 65: 766-776.
  • Huggins WM, Vu Nguyen T, Hahn NA, Baker JT, Kuo LG, Kaur D, Melander RJ, Gunn JS, Melander C. 2-Aminobenzimidazoles as antibiofilm agents against Salmonella enterica serovar Typhimurium. Medchemcomm. 2018 Sep 1; 9: 1547-1552.
  • González JF, Hahn MM, Gunn JS. Chronic biofilm-based infections: skewing of the immune response. Pathog Dis. 2018 Apr 1; 76:
  • Gunn JS, Ledford CH, Mousetes SJ, Grever MR. Biomedical Science Undergraduate Major: A New Pathway to Advance Research and the Health Professions. Teach Learn Med. 2018 Apr-Jun; 30: 184-192.
  • Moshiri J, Kaur D, Hambira CM, Sandala JL, Koopman JA, Fuchs JR, Gunn JS. Identification of a Small Molecule Anti-biofilm Agent Against Salmonella enterica. Front Microbiol. 2018; 9: 2804.
  • González JF, Alberts H, Lee J, Doolittle L, Gunn JS. Biofilm Formation Protects Salmonella from the Antibiotic Ciprofloxacin In Vitro and In Vivo in the Mouse Model of chronic Carriage. Sci Rep. 2018 Jan 9; 8: 222.

Biography

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

Biography

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

Biography

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major. Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

John S. Gunn, PhD, is a principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Children’s Hospital. He is also a professor in the Department of Pediatrics at The Ohio State University College of Medicine. Dr. Gunn is an associate director of the Ohio State Infectious Diseases Institute and the faculty director of the Biomedical Science Major.

Dr. Gunn received his undergraduate degree from Bowling Green State University and obtained his PhD from The University of Maryland. His postdoctoral studies were performed at Harvard Medical School and The University of Washington. The research program of the Gunn Lab studies host-microbe interactions in the gram-negative pathogens Salmonella and Francisella that lead to both acute and chronic disease. Their primary focus is determining the molecular mechanisms underlying chronic carriage of Salmonella Typhi in the gallbladder, which is mediated by biofilm formation on gallstones. Typhoid fever, caused by S. Typhi, is a global disease that disproportionally affects children.

Patient Care

Locations

Main Campus of Nationwide Children’s Hospital

Patient Care

Locations

Main Campus of Nationwide Children’s Hospital

Patient Care

Locations

Main Campus of Nationwide Children’s Hospital

Locations

Main Campus of Nationwide Children’s Hospital

Locations

Main Campus of Nationwide Children’s Hospital

Locations

Main Campus of Nationwide Children’s Hospital

  • Main Campus of Nationwide Children’s Hospital

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

Academic and Clinical Areas

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

Center for Microbial Pathogenesis

Principal Investigator

Primary Department

Center for Microbial Pathogenesis

  • Center for Microbial Pathogenesis
  • Principal Investigator
  • Primary Department
  • Center for Microbial Pathogenesis

Awards, Honors & Organizations

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

Awards, Honors & Organizations

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

Awards, Honors & Organizations

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016 Elected, Fellow, American Academy of Microbiology, 2016 CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014 Nominated, College of Medicine Distinguished Educator Award, The Ohio State University Editorial Board Member, Critical Reviews in Microbiology Member, American Society for Microbiology, 1988 - Present Member, International Endotoxin Society, 1988 - Present Member, American Gastroenterological Association, 1988 - Present Member, American Heart Association, 1988 - Present

  • Nominated, Distinguished Undergraduate Research Mentor of the Year, The Ohio State University, 2016
  • Elected, Fellow, American Academy of Microbiology, 2016
  • CMIB Outstanding Senior Faculty Mentor Award, The Ohio State University, 2014
  • Nominated, College of Medicine Distinguished Educator Award, The Ohio State University
  • Editorial Board Member, Critical Reviews in Microbiology
  • Member, American Society for Microbiology, 1988 - Present
  • Member, International Endotoxin Society, 1988 - Present
  • Member, American Gastroenterological Association, 1988 - Present
  • Member, American Heart Association, 1988 - Present

Education

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Education

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Education

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Date of Appointment at Nationwide Children’s Hospital: 11/01/2018

Graduate School

University of Maryland

Date Completed: 05/01/1993

Undergraduate School

Bowling Green State University

Date Completed: 05/01/1988

Professional Experience

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

Professional Experience

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

Professional Experience

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator2018 - Present The Ohio State University College of Medicine, Professor2017 - Present Infectious Diseases Institute, The Ohio State University, Associate Director2010 - Present The Ohio State University, Director, Biomedical Science Major2017 - 2018 The Ohio State University, Interim Chair, Department of Microbial Infection and Immunity

2018 - Present The Research Institute at Nationwide Children’s Hospital, Principal Investigator

Research Funding

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Research Funding

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Research Funding

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

NIH/NIAID Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator R01AI116917 06/01/2015-05/31/2020  The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage NIH/NIAID Virulence regulation of small RNAs in Francisella, Principal Investigator R21 AI140001  2/01/19-1/31/21  We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Mechanisms of the development and maintenance of Salmonella gallbladder carriage, Principal Investigator

R01AI116917

06/01/2015-05/31/2020 

The goal of this project is to determine both the host (gallbladder) and bacterial responses that allow carriage to develop and persist. This work will also further develop novel therapeutic interventions to prevent the development/maintenance of S. Typhi gallbladder carriage

Virulence regulation of small RNAs in Francisella, Principal Investigator

R21 AI140001

 2/01/19-1/31/21 

We examine the function of sRNAs in Francisella in regard to the regulation of virulence, and perform experiments to discover sRNAs and genes involved in intramacrophage survival (both host and bacterial genes/sRNAs/miRNAs).

Contact Information

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Contact Information

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Center for Microbial Pathogenesis

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)

Call us at: (614) 355-3403

Email John S Gunn

                    700 Children's DriveColumbus, OH 43205 (map)
  • Call us at:
  • (614) 355-3403
  • Email John S Gunn
  • 700 Children’s DriveColumbus, OH 43205 (map)

Connect with John Gunn

Connect on Twitter