The Histopathology Core provides exceptional services to internal investigators, including laboratory procedures in histology, electron microscopy (EM) and Atomic Force microscopy. Histology The Histopathology Core follows all required guidelines for CAP accreditation. Research support includes handling and processing of solid tissues with the following services:

Tissue fixation and paraffin embedding Paraffin block or frozen tissue sectioning (unstained slides and tissue scrolls) H&E and other chemical staining Direct and indirect immunohistochemical staining Selected special stains Serial sectioning RNase-free handling Tissue microarray construction TEM procedures including fixation, embedding, sectioning, and visualization

The Histopathology Core Laboratory is equipped with a variety of instrumentation to create and provide quality biospecimens for research.

Microtomes Cryostats Autostainer Perkin-Elmer TMA Master automated TMA arrayer MTA-1 manual Beecher TMA arrayer (2) Aperio whole slide scanners

Electron Microscopy (EM) The Histopathology Core provides services to obtain high resolution images of biological specimens.

Hitachi H-7650 Transmission Electron Microscope (TEM) with two digital cameras

Magnification ranges from 0.7K up to 200K AMtv542 imaging software

Hitachi S-4800 high resolution Scanning Electron Microscope (SEM)

Magnification ranges from 30x to 800,000x

Atomic Force Microscopy The Histopathology Core provides expertise in atomic force microscopy.

Asylum MFP-3D-BIO Atomic Force microscope

On an inverted Axioscope microscope specifically designed for biologists Attached to a Zeiss Apotome 2 to allow optical sectioning with fluorescent illumination Allows picoNewton force measurement, resolving the smallest features of a sample Fast Force mapping

Automated adhesion Elastic modulus analysis

Laser Dissection Microscopy The Histopathology Core provides microdissection of tissues on slides:

Zeiss LCM PALM Microbeam Laser Dissecting Scope with a pulsed UV laser (337nm) coupled to a Zeiss Axio microscope Laser capability

Micro-dissection and micro-ablation of small particles with sub micrometer precision

Leica DMI6000B inverted microscope with attached environmental chamber

Live cell imaging

Zeiss Axio microscope

Bright field Fluorescent illumination (IF) Time-lapse imaging

The Histopathology Core provides exceptional services to internal investigators, including laboratory procedures in histology, electron microscopy (EM) and Atomic Force microscopy.

Histology

The Histopathology Core follows all required guidelines for CAP accreditation. Research support includes handling and processing of solid tissues with the following services:

  • Tissue fixation and paraffin embedding
  • Paraffin block or frozen tissue sectioning (unstained slides and tissue scrolls)
  • H&E and other chemical staining
  • Direct and indirect immunohistochemical staining
  • Selected special stains
  • Serial sectioning
  • RNase-free handling
  • Tissue microarray construction
  • TEM procedures including fixation, embedding, sectioning, and visualization

The Histopathology Core Laboratory is equipped with a variety of instrumentation to create and provide quality biospecimens for research.

  • Microtomes
  • Cryostats
  • Autostainer
  • Perkin-Elmer TMA Master automated TMA arrayer
  • MTA-1 manual Beecher TMA arrayer (2)
  • Aperio whole slide scanners

Electron Microscopy (EM)

The Histopathology Core provides services to obtain high resolution images of biological specimens.

  • Hitachi H-7650 Transmission Electron Microscope (TEM) with two digital cameras
  • Magnification ranges from 0.7K up to 200K
  • AMtv542 imaging software
  • Hitachi S-4800 high resolution Scanning Electron Microscope (SEM)
  • Magnification ranges from 30x to 800,000x

Atomic Force Microscopy

The Histopathology Core provides expertise in atomic force microscopy.

  • Magnification ranges from 0.7K up to 200K

  • AMtv542 imaging software

  • Magnification ranges from 30x to 800,000x

  • Asylum MFP-3D-BIO Atomic Force microscope

  • On an inverted Axioscope microscope specifically designed for biologists

  • Attached to a Zeiss Apotome 2 to allow optical sectioning with fluorescent illumination

  • Allows picoNewton force measurement, resolving the smallest features of a sample

  • Fast Force mapping

  • Automated adhesion

  • Elastic modulus analysis

Laser Dissection Microscopy

The Histopathology Core provides microdissection of tissues on slides:

  • On an inverted Axioscope microscope specifically designed for biologists

  • Attached to a Zeiss Apotome 2 to allow optical sectioning with fluorescent illumination

  • Allows picoNewton force measurement, resolving the smallest features of a sample

  • Fast Force mapping

  • Automated adhesion

  • Elastic modulus analysis

  • Automated adhesion

  • Elastic modulus analysis

  • Zeiss LCM PALM Microbeam Laser Dissecting Scope with a pulsed UV laser (337nm) coupled to a Zeiss Axio microscope

  • Laser capability

  • Micro-dissection and micro-ablation of small particles with sub micrometer precision

  • Leica DMI6000B inverted microscope with attached environmental chamber

  • Live cell imaging

  • Zeiss Axio microscope

  • Bright field

  • Fluorescent illumination (IF)

  • Time-lapse imaging

  • Micro-dissection and micro-ablation of small particles with sub micrometer precision

  • Live cell imaging

  • Bright field

  • Fluorescent illumination (IF)

  • Time-lapse imaging